Therefore, this is a strong candidate for the Wld s mutation. No, create an account now. To confirm the presence of two extra repeats, the number of extra copies was estimated by PhosphorImager quantification of signal from genomic Southern blots. The contig is estimated to be 1. It also can be reproduced in vitro by using primary explants of dorsal root ganglion or superior cervical ganglion 5.
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DELL USB ENHANCED KEYBOARD P/N: KU 9985
There are very few examples of germ-line triplication, and little is known about the mechanism by which they might arise. Chen S, Bisby M A. No, create an account now. Please click here for more information. Roger Cox for the provision of P1 clones.
An kb tandem triplication in the slow Wallerian degeneration (Wlds) mouse
Our technology in the mass production of Nanofiber and naturally derived food formulation is unique and unattainable by our competitors. Second, if the gene crosses one or both boundaries of the triplicated region its coding sequence is likely to be disrupted.
Third, the coding region of the gene may be brought under the action of a new regulatory element by the DNA junctions formed in this rearrangement Fig. There were no discrepancies between the two assays. According to the model in Fig. Nanofiber Business Please click here for more information. For example, the introduction of a bcl-2 transgene or treatment with glial cell line-derived neurotrophic factor GDNF protects cell bodies in the motor neuron disease mouse pmn but, by themselves, these treatments do not prevent axonal loss iu do they alleviate the symptoms of the disease 10 This model raises the question of further repeat instability including partial or complete reversion.
Posthybridization washes were normally done at a stringency of 0. Hypotheses about what initiates Wallerian degeneration include loss of trophic support from the cell body and activation of calpain by calcium influx 34.
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Attempts to intervene in some neurological disorders will have to address degeneration both of 99855 axon and the cell body. Click here to join today!
Edited by Liane B. Visual inspection of this autoradiograph indicates that the shifted band is indeed more intense than the constant band, suggesting that there is more than one extra copy. Both the appearance of an additional fragment for probes located close to the junction Fig.
An kb Triplication in Wld s Mice. By continuing to use this site, you are consenting to our use of ky. Many end clones were placed on the genetic map by restriction fragment length polymorphism mapping to detect any chimerism although in fact none was encountered in these libraries and to locate recombination breakpoints. PhosphorImager analysis of two representative animals, WldA the same as in Fig. To confirm the presence of two extra repeats, the number of extra copies was estimated by PhosphorImager quantification of signal from genomic Southern blots.
The sequences reported in this paper have been deposited in the GenBank database accession nos.
First, probes from each end of the repeat unit detect Wld s -specific fragments of an identical size, despite being located at distant sites on the physical map. Packaged phage were used to infect LE cells. Interestingly, a duplication genotype was observed and there are kh plausible explanations for this.
The region whose copy number increased is substantially larger than 30 kb, because probes located across a kb region all gave a greater signal intensity but no difference in fragment size with a range of frequent-cutter restriction enzymes Fig. Clones spanning the proximal boundary of the repeat unit were isolated by genomic walking and the approximate location of the boundary deduced from hybridization patterns.
Support Center Support Center. Hence, the likely number of extra copies in these animals is two.
Unfortunately, it is not clear how to manipulate Wallerian degeneration because little is known about its regulation. Interestingly, five recombinations all map within a single P1 clone D23 at the proximal end of the candidate 995, indicating a hotspot of recombination. Hybridization signals were analyzed by using a Storm PhosphorImager and Molecular Dynamics version 4.
Genet Anal Tech Appl.